Composite
Part:BBa_K3729003
Designed by: Austin Wang, Julie Lin, Sinswien Lee Group: iGEM21_KCIS_NewTaipei (2021-10-09)
Strong promoter + strong rbs + BCD 1 gene + double terminator
The composite part consists of a strong promoter (BBa_J23100), a strong ribosome binding site(RBS), our BCD1 gene (BBa_K3729000), and a double terminator (BBa_K0015). This design of construct would lead to overexpression of the DNA sequence, further upregulating the production of butyryl- CoA dehydrogenase. Our composite part is synthesized by IDT.
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Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1
Illegal EcoRI site found at 113
Illegal SpeI site found at 230
Illegal SpeI site found at 1312 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal EcoRI site found at 113
Illegal NheI site found at 13
Illegal NheI site found at 36
Illegal SpeI site found at 230
Illegal SpeI site found at 1312 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
Illegal EcoRI site found at 113
Illegal BamHI site found at 684 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1
Illegal EcoRI site found at 113
Illegal SpeI site found at 230
Illegal SpeI site found at 1312 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1
Illegal EcoRI site found at 113
Illegal SpeI site found at 230
Illegal SpeI site found at 1312 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
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